Coding

Part:BBa_K2287022:Design

Designed by: Jiang Wu   Group: iGEM17_UCAS   (2017-10-19)


rhXOR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 612
    Illegal BamHI site found at 707
    Illegal BamHI site found at 848
    Illegal BamHI site found at 1617
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 957
    Illegal NgoMIV site found at 2079
    Illegal AgeI site found at 451
    Illegal AgeI site found at 2458
    Illegal AgeI site found at 2509
    Illegal AgeI site found at 3505
    Illegal AgeI site found at 3859
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1438
    Illegal SapI.rc site found at 1474
    Illegal SapI.rc site found at 2071


Design Notes

We considered the codon bias of E. coli and we obtained this part by gene synthesis.


Source

The original sequence comes from the genome of human, this codon optimized sequence comes from the paper "Human xanthine oxidase recombinant in E. coli: A whole cell catalyst for preparative drug metabolite synthesis"(M.F.Antunes et al. 2016)

References